How to solve access denied problem on IIS 7?

Two ways:

#1 Manual

In file explorer, right click, goto security tab, Add IIS APPPOOL\DefaultAppPool into your folder


#2 Command line:
icacls c:\inetpub\wwwroot\YourPath /grant "IIS APPPOOL\DefaultAppPool":(OI)(CI)(RX)

Reference: http://serverfault.com/questions/81165/how-to-assign-permissions-to-applicationpoolidentity-account